Metabolomics Analysis of Tuberculosis Drug Activity Using an Agilent 6545 Q-TOF LC/MS
نویسندگان
چکیده
Tuberculosis (TB) is both the leading cause of deaths due to an infectious disease and the leading cause of deaths due to a curable disease1. However, drug resistance1 is increasing while the pipeline of new drugs stagnates, and knowledge of existing drugs remains incomplete. Pyrazinamide (PZA) is a frontline TB drug1 whose mechanism of action remains among the most poorly understood. This Application Note presents a high-performance ion-pairing reversed-phase (IP-RP) Q-TOF LC/MS method that has enabled the biologically unbiased study of the impact of PZA on the Mycobacterium tuberculosis metabolome. Coupled with batch feature extraction and multivariate statistical analysis software, this workflow enabled the discovery of activity-specific metabolic changes that may help explain PZA’s unique metabolic effects.
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